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Three plasmids (pGL1124, pGL1224 and pGL1217) were produced to allow the alternative of 1 allele of CYC9Lister 427 cells. Trypanosomes had been transfected with Each individual plasmid individually, then subjected to your next transfection with among the other plasmids, with a unique resistance gene, with all pairwise combos executed.Research on th